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Identification of a rat liver cDNA and mRNA coding for the 28 kDa gap junction protein
Author(s) -
Heynkes Roland,
Kozjek Gisela,
Traub Otto,
Willecke Klaus
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80865-1
Subject(s) - complementary dna , messenger rna , identification (biology) , coding region , microbiology and biotechnology , biology , chemistry , biochemistry , gene , botany
By screening of a rat liver cDNA library with complex and deoxyinosine containing oligonucleotide probes a cDNA clone was isolated and shown by sequencing to code for the amino‐terminal half of the rat liver 28 kDa gap junction protein. The insert hybridized to a 1.9 kb species from rat and mouse liver poly(A) + RNA in Northern blot analysis. In embryonic mouse hepatocytes the amount of the 1.9 kb mRNA increased 3‐fold between 24 and 96 h in culture. This correlates with the previously described increase of the 28 kDa gap junction protein under these conditions.