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Specific binding of human growth hormone but not insulin‐like growth factors by human adipocytes
Author(s) -
DiGirolamo Mario,
Edén Staffan,
Enberg Gösta,
Isaksson Olle,
Lönnroth Peter,
Hall Kerstin,
Smith Ulf
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80856-0
Subject(s) - endocrinology , receptor , medicine , human placental lactogen , prolactin , human growth hormone , insulin , adipocyte , hormone , chemistry , growth hormone , biology , adipose tissue , placenta , fetus , pregnancy , genetics
Binding of human GH (hGH) and insulin‐like growth factors I and II (IGFI and II) to isolated human adipocytes from adult subjects was studied. Binding equilibrium for hGH at 24°C was reached at 120 min and half‐maximal specific binding at 6–8. Apparent K a was 2.1 × 10 9 M −1 and B max 7.3 × 10 −11 M/10 6 cells. The human fat cellgrowth hormone receptor recognized neither bovine, ovine or rat GH nor human prolactin or placental lactogen. No specific receptors for human IGFII could be demonstrated. Thus, human adipocytes do not possess IGF receptors but have specific GH receptors which recognize hGH but not GH from lower species.