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A single RNA species injected in Xenopus oocyte directs the synthesis of active tyrosine hydroxylase
Author(s) -
Horellou Philippe,
Guibert Bernard,
Leviel Vincent,
Mallet Jacques
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80854-7
Subject(s) - tyrosine hydroxylase , complementary dna , xenopus , tyrosine 3 monooxygenase , tyrosine , enzyme , biology , biochemistry , microbiology and biotechnology , gene , translation (biology) , rna , messenger rna , chemistry
Tyrosine hydroxylase, the rate limiting enzyme in the biosynthesis of catecholamine, is a tetramer composed of four subunits of the same molecular mass. A full length cDNA clone encoding tyrosine hydroxylase has been inserted into the SP6 expression system. Translation of the corresponding RNA in Xenopus oocyte results in enzymatic activity, demonstrating that a single gene contains all the necessary genetic information to code for a functional enzyme. The potential of this system in the analysis of posttranslational tyrosine hydroxylase modifications is discussed.