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31 P‐NMR studies of Mycoplasma gallisepticum cells using a continuous perfusion technique
Author(s) -
Egan William,
Barile Michael,
Rottem Shlomo
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80846-8
Subject(s) - glycolysis , intracellular , metabolism , phosphoenolpyruvate carboxykinase , biochemistry , fructose , intracellular ph , mycoplasma gallisepticum , nuclear magnetic resonance spectroscopy , sugar phosphates , biology , chemistry , mycoplasma , phosphate , enzyme , microbiology and biotechnology , stereochemistry
31 P‐NMR studies of Mycoplasma gallisepticum cells have been carried out using a continuous perfusion technique; these are the first such studies with this organism. Using this technique, glucose metabolism was monitored in the intact organisms, and cell extracts were prepared to identify the intermediates. Under glycolytic conditions, high levels of fructose‐1,6‐diphosphate were observed, indicating that this sugar may play a key role in the regulation of metabolism. The level of phospho enol pyruvate was low under normal glycolytic conditions, and did not increase during starvation. From the position of the internal inorganic phosphate peak, the intracellular pH was estimated. The cells were found to maintain an intracellular pH of ~ 7.1 over an investigated external pH range of 6.6–8.6.