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Effects of α‐amanitin on coordination of two mRNAs of ribulose‐bisphosphate carboxylase in greening pea leaves
Author(s) -
Sasaki Yukiko
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80828-6
Subject(s) - rubisco , protein subunit , specificity factor , ribulose 1,5 bisphosphate , chloroplast , messenger rna , greening , pyruvate carboxylase , biology , ribulose , transcription (linguistics) , microbiology and biotechnology , biochemistry , gene expression , photosynthesis , gene , enzyme , promoter , ecology , linguistics , philosophy
The effects of α‐amanitin on light induction of the two mRNAs of ribulose‐bisphosphate carboxylase/oxygenase (RuBisCO) in greening pea leaves were examined. Induction of the nuclear‐coded small‐subunit mRNA but not of the chloroplast‐coded large‐subunit mRNA was significantly reduced by α‐amanitin, indicating that the steady‐state level of the large‐subunit mRNA is not directly affected by that of the small subunit. This suggests that there is no direct link between nuclear transcription and light induction of the large‐subunit mRNA. The synthesis of RuBisCO was also slowed in proportion to the small‐subunit mRNA level, and there was not a large excess of the unassociated large subunit polypeptide. The synthesis of the two subunits is probably coordinated. Thus, the expression of the large subunit is partly controlled at the post‐transcriptional level.