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An infrequent generation of catenated network of pBR322 in Escherichia coli
Author(s) -
Komiyama Naoki,
Shishido Kazuo
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80826-2
Subject(s) - pbr322 , escherichia coli , plasmid , agarose gel electrophoresis , chemistry , monomer , dna , agarose , microbiology and biotechnology , biology , biochemistry , gene , polymer , organic chemistry
It was demonstrated that Escherichia coli infrequently generates the catenated network of pBR322. This complex pBR322 form was detected when DNA molecules could hardly enter the agarose gel during electrophoresis and was found to comprise monomers and dimers of the plasmid.