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Chilling followed by incubation at 37°C causes a reduction in NAD + levels which can be prevented by the poly(ADP‐ribose)transferase inhibitor 3‐aminobenzamide
Author(s) -
Cantoni Orazio,
Sestili Piero,
Cattabeni Flaminio,
Stocchi Vilberto
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80825-0
Subject(s) - nad+ kinase , incubation , enzyme , dna fragmentation , dna , chemistry , biochemistry , microbiology and biotechnology , fragmentation (computing) , transferase , dna damage , poly adp ribose polymerase , ribose , ethanol , biology , programmed cell death , apoptosis , ecology , polymerase
The exposure of cells for 60 min to a serum free medium at ice temperature followed by a return to normal culture conditions (30 min at 37°C) caused a dramatic decrease in NAD + levels. This decrease in NAD + was prevented by 3‐aminobenzamide. Alkaline elution analysis of DNA from cultures that were sisters to the ones utilized for measuring cellular NAD + content revealed an absence of DNA breakage. These data suggest that poly(ADP‐ribose)transferase may be induced in conditions not involving DNA fragmentation. The induction of this enzyme could therefore represent a cellular emergency reaction and not just a response to DNA damage.