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One‐ and two‐dimensional 90.5‐MHz 13 C‐NMR spectroscopy of the N ‐linked triantennary oligosaccharide units of calf fetuin
Author(s) -
Berman Elisha,
Bendel Peter
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80823-7
Subject(s) - anomer , fetuin , oligosaccharide , chemistry , nuclear magnetic resonance spectroscopy , galactose , chemical shift , spectroscopy , stereochemistry , crystallography , biochemistry , glycoprotein , physics , quantum mechanics
Complete assignments of all anomeric resonances in the proton and carbon spectra of the N ‐linked oligosaccharide units of fetuin were made using one‐ and two‐dimensional NMR spectroscopy. We are able to confirm the presence of microheterogeneity in the N ‐acetylneuraminic acid linkages to the galactose residues and the presence of a unique triantennary structure which carries a side chain: NeuAcα(1–3)Galβ(1–3) GlcNacβ(1–4)‐. Anomeric carbon chemical shifts changes resulting from long‐range conformational effects were observed.