Premium
Increased levels of cytochrome oxidase and sodium‐proton antiporter in the plasma membrane of Anacystis nidulans after growth in sodium‐enriched media
Author(s) -
Molitor Véronique,
Erber Walter,
Peschek Günter A.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80822-5
Subject(s) - antiporter , cytochrome c oxidase , sodium , biochemistry , chemistry , membrane , electron transport chain , cytochrome , oxidase test , cytochrome c , thylakoid , enzyme , mitochondrion , chloroplast , gene , organic chemistry
Typical rates of horse heart ferrocytochrome c oxidation by purified plasma membrane preparations of the cyanobacterium Anacystis nidulans after one week of growth at low (0.03 M) and high (0.4–0.5 M) Na + concentrations were 15 and 170per mg protein, respectively. Virtually no change was observed in the cytochrome oxidase activity of isolated and purified thylakoid membranes (approx. 3–5 nmol cytper mg protein irrespective of Na + concentration during growth). Concomitantly, the rate of whole cell respiration (oxygen uptake) and the capacity for Na + /H + exchange (per mg dry wt cells) by 0.4 M NaCl‐grown cells were 7‐ and 5‐fold enhanced, respectively, compared with control cells. The data are in accordance with electron transport‐driven Na + extrusion from respiring A. nidulans , primarily mediated by the plasma membrane‐bound cytochrome oxidase.