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Redox studies of the tetraheme cytochrome c 3 isolated from the propionate‐oxidizing, sulfate‐reducing bacterium Desulfobulbus elongatus
Author(s) -
Samain Eric,
Albagnac Guy,
Legall Jean
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80821-3
Subject(s) - chemistry , redox , cytochrome , cytochrome c , propionate , oxidizing agent , biochemistry , mitochondrion , enzyme , inorganic chemistry , organic chemistry
A tetraheme cytochrome c 3 was purified from extracts of Desulfobulbus elongatus . Redox potentiometry studies indicated that one of the four hemes has a uniquely high E m value (−30 mV), whereas the other three have a lower redox potential with E m values of −165 mV. In the presence of a membrane preparation from Db. elongatus the cytochrome c 3 is partially reduced (23% at pH 7.6) by succinate. The physiological significance of the high redox potential of this cytochrome c 3 is discussed as a function of the propionate metabolism of Desulfobulbus which is known to proceed via the succinate pathway.