Premium
Bacillus thuringiensis toxin inhibits K + ‐gradient‐dependent amino acid transport across the brush border membrane of Pieris brassicae midgut cells
Author(s) -
Sacchi V.Franca,
Parenti Paolo,
Hanozet Giorgio M.,
Giordana Barbara,
Lüthy Peter,
Wolfersberger Michael G.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80814-6
Subject(s) - brush border , bacillus thuringiensis , midgut , toxin , pieris brassicae , vesicle , biochemistry , biology , membrane , amino acid , membrane permeability , symporter , biophysics , microbiology and biotechnology , bacteria , larva , transporter , botany , genetics , gene
The luminal membrane of larval midgut cells is the site of action of insecticidal delta‐endotoxin from Bacillus thuringiensis . At concentrations that correspond to normal effective doses in vivo, the toxin inhibits the uptake of amino acids by brush border membrane vesicles prepared from midguts of Pieris brassicae larvae. The toxin does not interact with the K + ‐amino acid symport but rather increases the K + permeability of the membrane. The toxin does not increase the permeability of lepidopteran midgut brush border membrane to either Na + or H + nor does it increase the K + permeability of brush border membrane vesicles prepared from mammalian small intestine,