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Partial molecular characterization of endothelial cell mitogens from human brain: acidic and basic fibroblast growth factors
Author(s) -
Gautschi Peter,
Fràter-Schröder Marijke,
Böhlen Peter
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80812-2
Subject(s) - basic fibroblast growth factor , amino acid , fibroblast growth factor , fibroblast , cell growth , biochemistry , affinity chromatography , growth factor , peptide sequence , biology , sepharose , biological activity , human brain , cell , chemistry , receptor , gene , in vitro , enzyme , neuroscience
Two endothelial cell growth factors have been isolated from human brain extracts by a procedure involving heparin‐Sepharose affinity chromatography and reverse‐phase HPLC. On the basis of molecular size, amino acid composition and extended amino‐terminal sequences, the two proteins are structurally closely related to bovine acidic and basic fibroblast growth factors (aFGF, bFGF). The N‐terminal sequences of human and bovine aFGF diner in two positions while those for bFGF are indistinguishable. Human aFGF is microheterogenous: in addition to aFGF two N‐terminally truncated forms lacking the first and the seven first amino acids, respectively, were characterized. Human and bovine mitogens possess similar mitogenic activities.