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ATP‐induced calcium mobilization and inositol 1,4,5‐trisphosphate formation in H‐35 hepatoma cells
Author(s) -
Horstman Debra A.,
Tennes Karin A.,
Putney James W.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80809-2
Subject(s) - inositol , chemistry , calcium , mobilization , microbiology and biotechnology , inositol trisphosphate , adenosine triphosphate , biophysics , biochemistry , biology , receptor , organic chemistry , archaeology , history
Addition of ATP (but not epinephrine, angiotensin II, vasopressin, or platelet‐activating factor) to H‐35 hepatoma cells whose cellular lipids have been pre‐labelled with [ 3 H]inositol, causes a rapid increase in [ 3 H]inositol trisphosphate. In H‐35 cells pre‐incubated in the presence of 45 Ca 2+ , ATP causes a similarly rapid release of 45 Ca 2+ . The concentration‐effect relationships for inositol trisphosphate formation and Ca 2+ efflux are similar to those reported previously for differentiated hepatocytes. These results demonstrate that at least one of the Ca 2+ ‐mobilizing receptors normally found on hepatocytes is functionally retained in the H‐35 hepatoma cell line and thus could provide a useful model for the study of these receptor mechanisms in liver.