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Human purified interleukin‐1 inhibits DNA synthesis and cell growth of osteoblastic cell line (MC3T3‐E1), but enhances alkaline phosphatase activity in the cells
Author(s) -
Hanazawa Shigemasa,
Ohmori Yoshihiro,
Amano Shigeru,
Hirose Kimiharu,
Miyoshi Takehito,
Kumegawa Masayoshi,
Kitano Shigeo
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80758-x
Subject(s) - alkaline phosphatase , dna synthesis , cell culture , cell growth , microbiology and biotechnology , dna , osteoblast , chemistry , cell , biochemistry , cell division , biology , in vitro , enzyme , genetics
We examined the effects of human purified interleukin‐1 (IL‐1) on DNA synthesis, cell growth, and alkaline phosphatase activity in the osteoblastic cell line MC3T3‐E 1 under both preconfluent and confluent culture conditions. Addition of IL‐1 to the cells markedly inhibited their DNA synthesis and growth over the range 1–10. Such significant inhibitory effects were observed in cells cultivated in 1 or 5% fetal calf serum (FCS)‐containing alpha modification Eagle's medium (alpha‐MEM), but not in alpha‐MEM containing 10% FCS. In contrast, alkaline phosphatase activity was enhanced significantly by IL‐1 in the cell line cultivated in 1% FCS‐containing alpha‐MEM. These results demonstrate that human purified IL‐1 is effective in inducing the differentiation of osteoblastic cell MC3T3‐E1.