Premium
UV optical difference spectrum associated with the reduction of electron acceptor A 1 in photosystem I of higher plants
Author(s) -
Mansfield R.W.,
Evans M.C.W.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80747-5
Subject(s) - p700 , sodium dithionite , dithionite , photochemistry , photosystem i , electron acceptor , photosystem ii , chemistry , acceptor , electron paramagnetic resonance , electron transport chain , photosystem , electron transfer , absorbance , light harvesting complexes of green plants , quinone , electron , photosynthesis , nuclear magnetic resonance , physics , inorganic chemistry , stereochemistry , biochemistry , chromatography , quantum mechanics , enzyme , condensed matter physics
Photosystem I particles containing I P700 per 32 chlorophyll molecules were illuminated at cryogenic temperatures in the presence of sodium dithionite. Under conditions which specifically led to reduction of acceptor a 1 (as shown by its characteristic EPR spectrum) optical absorbance changes were detected between 240 and 325 nm. The appearance of these changes correlated closely with the increase in amplitude of the ai EPR signal. The possibility that a quinone‐like species is associated with, or directly involved in intermediary PS I electron flow is discussed.