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Flash‐induced absorption changes in photosystem I at low temperature: evidence that the electron acceptor A 1 is vitamin K 1
Author(s) -
Brettel K.,
Sétif P.,
Mathis P.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80746-3
Subject(s) - chemistry , photochemistry , photosystem i , absorption (acoustics) , absorption spectroscopy , electron acceptor , photosystem ii , acceptor , spinach , analytical chemistry (journal) , flash photolysis , photosynthesis , materials science , kinetics , biochemistry , reaction rate constant , chromatography , physics , composite material , condensed matter physics , quantum mechanics
Low temperature flash absorption spectroscopy has been applied to elucidate the chemical nature of the secondary electron acceptor A 1 of photosystem I (PS‐I). The flash‐induced absorption changes measured in digitonin‐fractionated spinach PS‐I particles at 10 K between 240 and 525 nm are shown to comprise a major decay phase with t~ 150 μs which has been attributed to the recombination reaction P‐700 + ·A 1 → P‐700·A 1 [(1984) Biochim. Biophys. Acta 767, 404‐414]. We present the absorption difference spectrum of this reaction and demonstrate that it contains contributions in the ultraviolet due to A 1 , which are characteristic of vitamin K 1 (phylloquinone).