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Isolation of a human ceruloplasmin cDNA clone that includes the N‐terminal leader sequence
Author(s) -
Mercer Julian F.B.,
Grimes Andrew
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80739-6
Subject(s) - ceruloplasmin , complementary dna , nucleic acid sequence , microbiology and biotechnology , peptide sequence , biology , oligonucleotide , complete sequence , amino acid , biochemistry , northern blot , sequence (biology) , southern blot , dna , gene , genome
A number of cDNA clones encoding human ceruloplasmin were identified using two mixed oligonucleotide probes. One of these clones was shown by DNA sequence analysis to span from the complete N‐terminal leader sequence to 114 amino acids short of the C‐terminus. The leader sequence consists of 19 primarily hydrophobic amino acids. Northern blot analysis of RNA from human liver showed two species of ceruloplasmin mRNA; a minor species of 3600 nucleotides and a major one of 4400 nucleotides.