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Inhibitory diffusible factor IDF45, a G 1 phase inhibitor
Author(s) -
Blat Christiane,
Chatelain Gilles,
Desauty Geneviéve,
Harel Louise
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80737-2
Subject(s) - dna synthesis , fast protein liquid chromatography , dna , stimulation , inhibitory postsynaptic potential , phase (matter) , cell cycle , chemistry , microbiology and biotechnology , chromatography , high performance liquid chromatography , biochemistry , cell , biology , endocrinology , organic chemistry
An inhibitory diffusible factor of 45 kDa (IDF45) was isolated from medium conditioned by dense cultures of 3T3 cells. The procedure involved Bio‐Gel PI 50 chromatography and 2 reverse‐phase FPLC. After the final step of purification, 60of IDF45 inhibited 50% of α‐globulin‐stimulated DNA synthesis. It was shown that IDF45 acted in the G 1 phase of the cell cycle. When added for 8 h in the G 1 phase of the cell cycle, it was able to inhibit DNA synthesis in the S phase which followed this G 1 phase. Furthermore, IDF45 inhibited the early stimulation of RNA synthesis induced by α‐globulin.