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Promotor and enhancer like activity at the 5'‐end of normal and T24 Ha‐ ras 1 genes
Author(s) -
Spandidos Demetrios A.,
Riggio Marcello
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80736-0
Subject(s) - enhancer , chloramphenicol acetyltransferase , gene , promoter , microbiology and biotechnology , transfection , biology , exon , coding region , transcription (linguistics) , genetics , gene expression , linguistics , philosophy
We have used a short‐term transfection technique, in which we monitor the ability of DNA fragments to induce the expression of the chloramphenicol acetyltransferase (CAT) gene in rat 208F fibroblast cells. Using appropriate vectors we have assayed for promoter or enhancer activity of the 0.8 kb SstI fragment located within the 5'‐flanking sequences of the first coding exon of the human T24 and normal Ha‐ ras 1 genes. We find that this fragment contains promoter and enhancer activities in both the normal and the T24 Ha‐ ras 1 gene.