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Putative ammo‐terminal presequence for β‐subunit of plant mitochondrial F 1 ATPase deduced from the amino‐terminal sequence of the mature subunit
Author(s) -
Kobayashi Kazuto,
Iwasaki Yukimoto,
Sasaki Takuji,
Nakamura Kenzo,
Asahi Tadashi
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80731-1
Subject(s) - protein subunit , amino acid , biochemistry , peptide sequence , atpase , biology , nucleic acid sequence , mitochondrion , specificity factor , homology (biology) , microbiology and biotechnology , gene , rna , enzyme , rna dependent rna polymerase
The α‐ and β‐subunits of sweet potato mitochondrial F 1 ATPase were purified from the F 1 complex by gel filtration and ion‐exchange high‐performance liquid chromatography. Isoelectric focussing and N‐terminal amino acid sequencing indicated that the purified β‐subunit contains at least two polypeptides similar to each other. The N‐terminal 18 amino acid sequence of the β‐subunit showed homology to the amino acid sequence of the tobacco mitochondrial F 1 ATPase β‐subunit precursor deduced from the nucleotide sequence [(1985) EMBO J. 4, 2159‐2165] between residues 56 and 73, suggesting that the N‐terminal 55 amino acids of the tobacco precursor constitute the presequence required for mitochondrial targetting.