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Cloning of rat brain protein kinase C complementary DNA
Author(s) -
Ono Yoshitaka,
Kurokawa Tsutomu,
Kawahara Kenji,
Nishimura Osamu,
Marumoto Ryuji,
Igarashi Koichi,
Sugino Yukio,
Kikkawa Ushio,
Ogita Kouji,
Nishizuka Yasutomi
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80724-4
Subject(s) - complementary dna , cdna library , nucleic acid sequence , microbiology and biotechnology , cloning (programming) , biology , peptide sequence , clone (java method) , oligonucleotide , amino acid , homology (biology) , protein kinase a , dna , molecular cloning , biochemistry , kinase , gene , computer science , programming language
Four peptides derived from rat brain protein kinase C were partially sequenced. Using synthetic oligonucleotides deduced from the amino acid sequences as probes, a clone of complementary DNA (cDNA) was isolated from a cDNA library prepared from the same tissue. The nucleotide sequence of this cDNA clone revealed the primary structure of the carboxyl‐terminal region as having 224 amino acids, with significant sequence homology with cyclic AMP‐dependent and cyclic GMP‐dependent protein kinases.