Premium
L‐(+)‐Tartrate dehydratase from Pseudomonas putida is an iron‐sulphur enzyme
Author(s) -
Kelly Janice M.,
Scopes Robert K.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80700-1
Subject(s) - dehydratase , pseudomonas putida , chemistry , enzyme , biochemistry , ligand (biochemistry) , sulfur , enzyme assay , stereochemistry , organic chemistry , receptor
The enzyme L‐(+)‐tartrate dehydratase has been isolated from extracts of Pseudomonas putida by a one‐step procedure involving dye‐ligand chromatography. The enzyme loses activity rapidly in the absence of Fe 2+ ; concentrated solutions have a brown colour typical of iron‐sulphur proteins. Analysis of iron and acid‐labile sulphide indicated 3–5 atoms of each per molecule of 100 kDa. The enzyme's structure consists of four subunits, two each of 23 and 27 kDa.