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Electrogenic reduction of the secondary quinone acceptor in chromatophores of Rhodospirillum rubrum
Author(s) -
Kaminskaya Olga P.,
Drachev Lel A.,
Konstantinov Alexander A.,
Semenov Alexey Yu.,
Skulachev Vladimir P.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80691-3
Subject(s) - rhodospirillum rubrum , electron transfer , chemistry , rhodospirillales , chromatophore , flash (photography) , photochemistry , rhodospirillaceae , protonation , kinetics , electron acceptor , biophysics , stereochemistry , biochemistry , photosynthesis , biology , enzyme , organic chemistry , physics , optics , ion , quantum mechanics , fishery
Electron transfer Q A → Q B has been reconstituted with added Q‐10 in Rhodospirillum rubrum chromatophores associated with a phospholipid‐impregnated collodion film. Rapid kinetics measurements of laser flash‐induced ΔΨ generated in the chromatophores show that whereas electron transfer from Q a − to Q B upon the first flash is not electrogenic in dark‐adapted chromatophores, reduction of Q − B to Q b h 2 induced by the second flash gives rise to an electrogenic phase with τ = 250 μs at pH 7.5 which contributes about 10% to the total ΔΨ generated upon the flash. The electrogenic phase is ascribed to vectorial protonation of Q 2− B .