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Thyrotropin and norepinephrine stimulate the metabolism of phosphoinositides in FRTL‐5 thyroid cells
Author(s) -
Philp Nancy J.,
Grollman Evelyn F.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80685-8
Subject(s) - phosphatidylinositol , endocrinology , adenylate kinase , medicine , stimulation , cyclase , metabolism , phospholipid , chemistry , receptor , enzyme , signal transduction , biology , biochemistry , membrane
Hormone‐induced changes in phospholipid metabolism were examined in a functioning rat thyroid cell line (FRTL‐5). Stimulation of FRTL‐5 cells, prelabeled with 32 P, with TSH or NE resulted in a rapid decrease in the radioactivity of both phosphatidylinositol 4,5‐bisphosphate (PIP 2 ) and phosphatidylinositol 4‐mono‐phosphate (PIP). The effects of TSH on phospholipid metabolism and calcium mobilization are independent of those on adenylate cyclase. This suggests that the TSH receptor may be unique in that it activates enzyme cascades involved in cAMP production and Ca 2+ mobilization.