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[Ser 7 ]neurotensin: isolation from guinea pig intestine
Author(s) -
Shaw Christopher,
Thim Lars,
Conlon J.Michael
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80684-6
Subject(s) - neurotensin , guinea pig , residue (chemistry) , antiserum , chemistry , peptide , neuropeptide , oligopeptide , biochemistry , biology , microbiology and biotechnology , antibody , endocrinology , immunology , receptor
Using three antisera to neurotensin of defined regional specificity, a novel neurotensin has been identified in extracts of guinea pig brain and small intestine. The primary structure of the peptide was established as: pGlu Leu Tyr GIu Asn Lys Ser Arg Arg Pro Tyr He Leu. Guinea pig neurotensin differs from bovine neurotensin by substitution of a prolyl residue by a seryl residue at position 7. Synthetic [Ser 7 ]neurotensin showed identical Chromatographie and immunochemical properties to guinea pig neurotensin. This difference in primary structure may account for some of the anomalous pharmacological effects of bovine neurotensin on guinea pig tissues.