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Role of catalase in metabolism of hydrogen peroxide by the perfused rat heart
Author(s) -
Thayer William S.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80664-0
Subject(s) - catalase , hydrogen peroxide , metabolism , chemistry , biochemistry , oxidative metabolism , medicine , enzyme
Cardiac metabolism of H 2 O 2 was studied by determining the concentration dependence for H 2 O 2 ‐stimulated release of GSSG, an indicator for flux through the glutathione peroxidase pathway, in perfused heart preparations. Treatment of rats with aminotriazole in vivo inhibited heart catalase by 83% and shifted the doseresponse curve for GSSG release toward lower H 2 O 2 concentrations. In aminotriazole‐treated rats, 50 μM H 2 O 2 elicited a maximal rate of GSSG release (about 5 nmol per g heart), whereas 200 μM H 2 O 2 was necessary for obtaining a similar rate of GSSG release in control rat hearts. The results show that catalase, although present at low levels of activity in the heart compared to other organs, functions as a major route for detoxication of H 2 O 2 in the myocardium.