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Effects of some substrate analogs on aerobactin synthetase from Aerobacter aerogenes 62‐1
Author(s) -
Appanna Vasu D.,
Viswanatha Thammaiah
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80658-5
Subject(s) - chemistry , aerobactin , substrate (aquarium) , hydroxylysine , citric acid , propionate , enzyme , biochemistry , stereochemistry , amino acid , enterobacteriaceae , biology , lysine , ecology , escherichia coli , gene
Tricarballylic acid was the only analog of citric acid that appeared to be a substrate for aerobactin synthetase. The others, succinic acid, glutaric acid and 3‐methyl‐3‐hydroxyglutaric acid, though potent inhibitors of aerobactin synthesis, were neither activated nor utilised by the enzyme system. Aerobactin synthetase seemed to mediate the condensation of either N 6 ‐ n ‐butyryl‐ N 6 ‐hydroxylysine, or N 6 ‐succinyl‐ N 6 ‐hydroxylysine, or N 6 ‐acetyllysine and citric acid, in a manner analogous to that of the natural substrate, N 6 ‐acetyl‐ N 6 ‐hydroxylysine.