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Malic enzyme levels are increased by the activation of NADPH‐consuming pathways: detoxification processes
Author(s) -
Ayala Antonio,
F-Lobato María,
Machado Alberto
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80657-3
Subject(s) - detoxification (alternative medicine) , malic enzyme , enzyme , chemistry , biochemistry , metabolic pathway , enzyme activator , medicine , alternative medicine , pathology , dehydrogenase
The administration to rats of either t ‐butyl hydroperoxide or phénobarbital, compounds that are metabolized through detoxification processes, produces an increase in specific activity of the NADPH‐consuming enzymes, glutathione reductase and NADPH‐cytochrome c reductase. These compounds also produce a very significant increase in the specific activity of malic enzyme. Immunoprecipitation with a specific antibody for malic enzyme indicates that specific activity changes are the result of corresponding changes in the amounts of enzyme protein present. The administration of 1,3‐bis(chloroethyl(‐1‐nitrosourea (a glutathione reductase inhibitor) together with t ‐butyl hydroperoxide abolishes any stimulation of malic enzyme activity. These results indicate that an increase in NADPH consumption induces the synthesis of malic enzyme. Alternatively, a protection of enzyme degradation cannot be rigorously excluded.