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Molecular cloning of cDNA of S100α subunit mRNA
Author(s) -
Kuwano Ryozo,
Maeda Toshinaga,
Usui Hiroshi,
Araki Kazuaki,
Yamakuni Toru,
Ohshima Yuka,
Kurihara Tadashi,
Takahashi Yasuo
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80656-1
Subject(s) - complementary dna , messenger rna , polyadenylation , microbiology and biotechnology , coding region , cloning (programming) , molecular cloning , biology , protein subunit , northern blot , gene , genetics , computer science , programming language
The primary structure of the bovine S‐100α mRNA on the basis of molecular cloning and sequence analysis of the cDNA are described. The sequence is composed of 532 bp which include the 282 bp of the complete coding region, 89 bp at the 5'‐noncoding region, 161 bp at the 3'‐noncoding region, polyadenylation signal, ATTAAA and poly(A) tail. Northern blot analysis shows that the size of S‐100α mRNA is about 700–800 bases long and a single mRNA occurs in bovine brain. Bovine brain contains both S100α and β subunits and their mRNAs. In contrast, the rat brain contains only S 100α subunit and its mRNA.