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Monoclonal antibody detects Ag polymorphism of apolipoprotein B
Author(s) -
Tikkanen Matti J.,
Ehnholm Christian,
Bütler Rene,
Young Stephen G.,
Curtiss Linda K.,
Witztum Joseph L.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80648-2
Subject(s) - apolipoprotein b , epitope , monoclonal antibody , microbiology and biotechnology , antibody , chemistry , allele , biology , monoclonal , biochemistry , genetics , gene , cholesterol
A monoclonal antibody (MB‐19) was used to investigate the polymorphism of apolipoprotein B in a large family and in unrelated subjects. Apolipoprotein B was shown to exhibit high‐, intermediate‐ or low‐affinitybinding to this antibody. Thus, MB‐19 bound strongly to the Ag(c) epitope, an Ag antigenic domain previously characterized by human antisera, while it bound only weakly to the allelic epitope Ag(g). It proved therefore useful for the detection of the two corresponding allelic apoB species designated apoB c (high‐affinity binding) and apoB g (low‐affinity binding), and for confirming their co‐dominant transmission. Intermediate binding resulted from the presence of a mixture of both apoB populations in heterozygous subjects.