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Specific lysis of noradrenergic synaptosomes by an antiserum to dopamine‐β‐hydroxylase
Author(s) -
Docherty Maureen,
Bradford H.F.,
Joh T.H.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80644-5
Subject(s) - antiserum , lysis , tyrosine hydroxylase , dopamine , chemistry , endocrinology , medicine , adrenal medulla , biochemistry , microbiology and biotechnology , biology , antibody , catecholamine , immunology
An antiserum to dopamine‐β‐hydroxylase purified from bovine adrenal medulla, acting in the presence of complement, caused the release of 12% of lactate dehydrogenase, 20% of tyrosine hydroxylase activity, and 40% of noradrenaline (NA) content from synaptosomes prepared from rat brain cerebral cortex. Uptake of [ 3 H]NA was reduced by 54%. Anti‐serum alone or complement alone were without action. The antiserum plus complement had no effect on choline uptake and did not release choline acetyltransferase, glutamate decarboxylase, dopamine or 5‐hydroxytryptamine. These results suggest selective lysis of noradrenergic terminals had occurred. An enhancement of lysis was not observed when synaptosomes were stimulated with 75 K + and exposed to a sub‐maximal dose of antiserum, plus complement.