Premium
Monoclonal antibodies against GA 13 ‐imide recognize the endogenous plant growth regulator, GA 4 , and related gibberellins
Author(s) -
Eberle J.,
Yamaguchi I.,
Nakagawa R.,
Takahashi N.,
Weiler E.W.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80642-1
Subject(s) - monoclonal antibody , imide , chemistry , subclass , antibody , conjugate , gibberellin , microbiology and biotechnology , biochemistry , stereochemistry , biology , organic chemistry , genetics , immunology , mathematical analysis , mathematics
Monoclonal antibody(MAB)‐secreting hybridomas were selected from fusions of spleen cells derived from Balb/c mice immunized with the BSA conjugate of GA 13 ‐19,20‐imide‐β‐alanine 7‐methyl ester with the myeloma line X63.Ag8.653. Selection based on [1,2‐ 3 H]GA 4 methyl ester binding capacity yielded two stable hybridoma clones that secreted antibodies of the IgGl subclass. These MAB exhibited high affinities for GA 4 methyl ester (3.2 × 10 8 and 1.0 × 10 9 M −1 ) which allowed quantitation by HPLC‐RIA of nanogram or sub‐nanogram amounts of the gibberellins A 2 , A 3 , A 4 A 7 and A 9 as the methyl esters, in biological fluids.