Premium
Cross‐reactivity studies on the interaction between elongation factors Tu and Ts from Streptomyces aureofaciens and Escherichia coli in the GDP exchange reaction
Author(s) -
Weiser Jaroslav,
Ruusala Tarmo
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80638-x
Subject(s) - elongation , streptomyces aureofaciens , reactivity (psychology) , escherichia coli , chemistry , elongation factor , biochemistry , biophysics , biology , streptomyces , bacteria , genetics , materials science , gene , medicine , ribosome , rna , alternative medicine , pathology , metallurgy , ultimate tensile strength
The method of purification of elongation factor Ts from Streptomyces aureofaciens is described. Purified elongation factors Ts from S. aureofaciens and Escherichia coli were tested in cross‐reactivity studies with elongation factors Tu from both species in a GDP exchange reaction under equilibrium and non‐equilibrium conditions. Experiments have revealed that slower spontaneous release of GDP from S. aureofaciens EF‐Tu is compensated for by higher affinity of homologous EF‐Ts towards EF‐Tu and thus the initial rates of EF‐Ts catalysed GDP exchange can be kept the same in both E. coli and S. aurefaciens in vitro systems.