Premium
Immunochemical study of the plasma low and high density lipoproteins in Tangier disease
Author(s) -
Dumon M.F.,
Visvikis S.,
Manabe T.,
Clerc M.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80591-9
Subject(s) - tangier disease , apolipoprotein b , lipoprotein , chemistry , electrophoresis , high density lipoprotein , polyacrylamide gel electrophoresis , chromatography , cholesterol , biochemistry , abca1 , enzyme , transporter , gene
Major disturbances of the lipoproteins in Tangier serum have been investigated using electrophoretic and immunochemical techniques. Previously described anomalies concerning the striking deficiency in HDL and the very low levels of apo A‐I and apo A‐II in Tangier patients are illustrated and explained. Anomalies concerning the fast LDL of Tangier serum are attributed to different forms of apo B not previously described. These data are strengthened by the features of a 2‐dimensional electrophoresis method elaborated in the laboratory which allows apoproteins to separate in the second dimension. These apoproteins are obtained by the delipidation of the lipoproteins fractionated in a first polyacrylamide discontinuous gel. This method clearly shows the distribution of apoproteins in the first lipoprotein track and is in perfect accordance with the new concept of lipoprotein particles.