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Glucagon‐stimulated respiration and intracellular Ca 2+
Author(s) -
Kraus-Friedmann Naomi
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80585-3
Subject(s) - glucagon , extracellular , intracellular , respiration , stimulation , endocrinology , medicine , chemistry , hormone , biology , biochemistry , anatomy
The effects of extra‐ and intracellular Ca 2+ on glucagon‐stimulated respiration were examined in perfused rat liver. Glucagon increased the uptake of O 2 to a significantly greater extent in Ca 2+ ‐containing perfusate than in Ca 2+ ‐free perfusate. If, however, the livers were perfused first with Ca 2+ ‐containing perfusate for 60 min in order to load the hormone‐sensitive Ca 2+ pool(s) and subsequently with Ca 2+ ‐free perfusate, glucagon was able to stimulate O 2 uptake to the same extent in Ca 2+ ‐free, as in Ca 2+ ‐containing perfusate. These experiments support previous observations of a connection between Ca 2+ and the hormonal stimulation of respiration, but indicate a role for intracellular, rather than extracellular, Ca 2+ in the process.