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Adenosine transporters in chromaffin cells
Author(s) -
Torres M.,
Molina P.,
Miras-Portugal M.T.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80583-x
Subject(s) - adenosine , transporter , chromaffin cell , adenosine receptor , chemistry , receptor , medulla , adrenal medulla , biophysics , microbiology and biotechnology , medicine , endocrinology , biochemistry , biology , gene , catecholamine , agonist
Chromaffin cells from bovine adrenal medulla are a useful model to approach adenosine transport and metabolism in neural cells. Dipyridamol has been shown to be an adenosine transport inhibitor with high affinity. To quantify the adenosine transporters a labelled dipyridamol analogue, [ 14 C]dipyridamol acetate, was synthesized. This compound had a K i = 5.3 ± 0.43 nM according to the Dixon method, and 4.58 ± 0.46 nM when the receptor number molarity was taken into account showing, like dipyridamol, a non‐competitive mechanism. The high‐affinity receptors present in chromaffin cells showed a K d = 6.8 ± 0.8 nM and the receptor number was 630000 ± 40000 per cell.