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Characterization of new gangliosides of the lactotetraose series in murine xenografts of a human glioma cell line
Author(s) -
Månsson J.-E.,
Fredman P.,
Bigner D.D.,
Molin K.,
Rosengren B.,
Friedman H.S.,
Svennerholm L.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80580-4
Subject(s) - sialic acid , galactose , linkage (software) , cell culture , glioma , n acetylneuraminic acid , biochemistry , chemistry , biology , amino acid , microbiology and biotechnology , genetics , gene
The major mono–and disialogangliosides of the extensively characterized established human glioma line D54MG were isolated and purified from subcutaneous solid xenografts grown in athymic (nu/nu) mice. Structural determination showed that they belonged to the lactotetraosylceramide series. The sialyllactotetraosylceramide contained 90% N –glycolyl– and 10% N –acetylneuraminic acid linked in an α2–3 linkage (IV 3 NeuGc–LcOse 4 Cer, IV 3 NeuAc–LcOse 4 Cer). The disialogangliosides had a previously undescribed type of structure with sialic acids linked to the terminal galactose in an α2–3 linkage and to N –acetylglucosamine in an α2–6 linkage. Not only did species with NeuAc or NeuGc occur, but also species with mixtures of the two sialic acids, e.g. NeuAC and NeuGc. The schematic structures of the new disialogangliosides are