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Characteristics of two types of legumin genes in the field bean ( Vicia faba L.var. minor) genome as revealed by cDNA analysis
Author(s) -
Wobus Ulrich,
Bäumlein Helmut,
Bassüner Ronald,
Heim Ute,
Jung Rudolf,
Müntz Klaus,
Saalbach Gerhard,
Weschke Winfriede
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80573-7
Subject(s) - legumin , subfamily , biology , vicia faba , complementary dna , genetics , gene , homology (biology) , genome , botany , storage protein
Nucleotide sequence analysis of cDNA clones coding for field bean legumin precursor polypeptides revealed two different types, called A and B. Although homologous, both types differ in several sequence characteristics. Comparison with similar data from soybean and recent findings from pea leads to the following conclusions: (i) the two types of legumin genes described represent two subfamilies, A and B, which are probably of widespread occurrence; (ii) legumin genes or subunits can best be placed in either subfamily A or B by sequence homology, in addition B‐type subunits contain generally fewer (or none at all in V. faba ) Met residues as compared to A‐type subunits; (iii) members of one subfamily from different species are more homologous than members of either subfamily within a species, therefore the two subfamilies must have arisen long before speciation of the genera Glycine, Pisum and Vicia ; (iv) during speciation members of the B‐subfamily diverged significantly more than members of the A‐subfamily.