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Isolation and characterization of variant IGF‐1 as well as IGF‐2 from adult human brain
Author(s) -
Carlsson-Skwirut Christine,
Jörnvall Hans,
Holmgren Arne,
Andersson Charlotte,
Bergman Tomas,
Lundquist Gunilla,
Sjögren Barbro,
Sara Vicki R.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80568-3
Subject(s) - fast protein liquid chromatography , human brain , neuropeptide , peptide , high performance liquid chromatography , size exclusion chromatography , somatomedin , chemistry , affinity chromatography , biochemistry , biology , chromatography , growth factor , neuroscience , receptor , enzyme
The forms of somatomedin present in the adult human brain have been characterized in this study. Two peptides were purified by acidification, size exclusion chromatography, affinity chromatography, FPLC and HPLC. structural analysis identified these peptides as the variant form of IGF‐1 with a truncated N‐terminal region earlier isolated from human fetal brain and IGF‐2. The presence of the truncated IGF‐1 variant and IGF‐2 in the human CNS suggests their role as neuropeptides.