Premium
Reversible photochemical holeburning in Rhodopseudomonas viridis reaction centers
Author(s) -
Boxer Steven G.,
Middendorf Thomas R.,
Lockhart David J.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80545-2
Subject(s) - excited state , photochemistry , ion , absorption band , chemistry , excitation , wavelength , absorption (acoustics) , ground state , atomic physics , photosynthetic reaction centre , electron transfer , materials science , physics , optoelectronics , optics , organic chemistry , quantum mechanics
The change in absorption of the band assigned to the primary electron donor in R. viridis reaction centers (P960) has been measured at 1.4–2.1 K following narrow bandwidth excitation at many wavelengths within the P960 absorption band. In all cases nearly the entire band was photobleached (full width at half‐maximum about 400 cm −1 ). This result can be explained by either an ultra‐fast decay process from the initially excited state or a substantial difference in the equilibrium nuclear configuration between the ground and excited state of the donor. The latter may arise if the excited state of the donor has substantial charge transfer character. The results suggest a charge separation step which precedes formation of a bacteriopheo‐phytin b anion.