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Volume‐dependent and NEM‐stimulated K + Cl −1 transport is elevated in oxygenated SS, SC and CC human red cells
Author(s) -
Canessa Mitzy,
Spalvins Anda,
Nagel Ronald L.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80538-5
Subject(s) - bumetanide , cotransporter , efflux , ouabain , chemistry , biophysics , symporter , ion transporter , transporter , swelling , intracellular , membrane transport , biochemistry , microbiology and biotechnology , sodium , biology , membrane , organic chemistry , gene , chemical engineering , engineering
Mechanisms involved in cell volume regulation are important in SS, SC cells as they might be involved in determining the extent of sickling and the generation of dense cells and irreversibly sickled cells. We have studied in these cells the response to cell swelling of the K + Cl − transporter. We found that Hb SS, SC and CC red cells have higher values of a ouabain‐resistant, chloride‐dependent and NEM‐stimulated K + efflux than AA red cells. In contrast, the Na + K + Cl − cotransport estimated from the bumetanide‐sensitive component of K + efflux was not significantly different in SS, SC and CC red cells. The (ouabain + bumetanide)‐resistant K + efflux from SS, SC and CC red cells was stimulated by cell swelling induced by reduction of the osmotic pressure (300 to 220 ) and pH (8 to 7) of the flux media (140 mM NaCl). The Cl − ‐dependent K + efflux stimulated by osmotic swelling highly correlated with the NEM‐stimulated component ( r = 0.8, p < 0.001, n = 22) and the acid‐pH‐induced swelling ( r = 0.969, p < 0.001, n = 22), indicating that it is driven by the K + Cl − transporter.