Premium
fixA, B and C genes are essential for symbiotic and free‐living, microaerobic nitrogen fixation
Author(s) -
Gubler Marcel,
Hennecke Hauke
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80536-1
Subject(s) - azotobacter vinelandii , nitrogen fixation , bradyrhizobium japonicum , diazotroph , biology , mutant , bradyrhizobium , gene , genetics , microbiology and biotechnology , symbiosis , nitrogenase , rhizobiaceae , bacteria
Site‐specific mutations were contructed within Bradyrhizobium japonicum fixA, B and C regions that had previously been identified by interspecies hybridization [(1985) Mol.Gen.Genet. 199, 315‐322]. The corresponding mutants were not only Fix − in otherwise fully developed soybean root nodules but also Nif − in free‐living microaerobic culture. Specific fixA, B and C probes hybridized to DNA of the aerobic diazotroph, Azotobacter vinelandii . We hypothesize, therefore, that fixA, B and C genes are of general importance for aerobic/microaerobic nitrogen fixation.