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Lipogenesis in isolated human sebaceous glands
Author(s) -
Cassidy D.M.,
Lee C.M.,
Laker M.F.,
Kealey T.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80533-6
Subject(s) - lipogenesis , valine , isoleucine , chemistry , squalene , leucine , cholesterol , biochemistry , medicine , endocrinology , amino acid , metabolism , biology
Lipogenesis in isolated human sebaceous glands from [U‐ 14 C]glucose, [U‐ 14 C]leucine, [U‐ 14 C]isoleucine, and [U‐ 14 C]valine has been determined by thin‐layer chromatography. Total lipogenesis from 2 [U‐ 14 C]glucose was 114.8 ± 22.3 per h (mean ± SE), with 53.8% being incorporated into triglycerides, 20.2% into squalene, 12.8% into phospholipids, 2.1% into cholesterol and 7.1% into wax monoester and cholesterol ester and 5% into di‐ and monoglycerides and free fatty acids. Total lipogenesis from 2 [U‐ 14 C]leucine, 2 mmo [U‐ 14 C]isoleucine, and 2 [U‐ 14 C]valine in the presence of 2 glucose was 26, 29 and 9%, respectively, of that seen with 2 glucose alone. The pattern of 14 C distribution in the various lipid classes from the three U‐ 14 C‐labelled branched‐chain amino acids was not significantly different from that seen with [U‐ 14 C]glucose.