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Synthesis of cell‐impermeable inhibitor of phospholipase A 2
Author(s) -
Yedgar Saul,
Reisfeld Nurit,
Dagan Arie
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80531-2
Subject(s) - intracellular , cell , extracellular , organelle , membrane , chemistry , cell membrane , microbiology and biotechnology , phospholipase a , secretion , biochemistry , phospholipase c , phospholipase , phospholipase a2 , enzyme , biophysics , biology
Phospholipase A 2 (PLA 2 ) in the cell surface membrane is considered a regulator of cellular secretion. The distinction between the role of the cell surface and the intracellular PLA 2 is not clear, since it has not been possible to differentiate unequivocally the activity of the enzymes in the various organelles. The use of an extracellular inhibitor of PLA 2 can greatly contribute to the understanding of cell surface PLA 2 function. In this paper, the preparation of a cell‐impermeable inhibitor of PLA 2 is presented. This substance incorporates into lipid membranes and is capable of blocking the hydrolysis of membrane phospholipids by snake venom as well as by cell membrane PLA 2 .