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Direct photoaffinity labeling of soluble GTP‐binding proteins in Dictyostelium discoideum
Author(s) -
Schleicher Michael,
Witke Walter,
Isenberg Gerhard
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80529-4
Subject(s) - dictyostelium discoideum , gtp' , photoaffinity labeling , biochemistry , chemistry , size exclusion chromatography , nucleotide , gtp binding protein regulators , cytoplasm , binding site , dictyostelium , molecular mass , g protein , receptor , enzyme , gene
Major cytoplasmic GTP‐binding proteins in Dictyostelium discoideum were identified by direct photoaffinity labeling with [α‐ 32 P]GTP. Three actin‐binding proteins and a protein with an apparent molecular mass of 24 kDa (p24) could be labeled with [α‐ 32 P]GTP. p24 binds to DEAE‐cellulose, behaves like a monomer during gel filtration and was purified to homogeneity by GTP‐affinity chromatography. In comparison to other nucleotide triphosphates the binding of GTP to p24 is highly specific.