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Energy‐linked cardiac transport system for glutathione disulfide
Author(s) -
Ishikawa Toshihisa,
Zimmer Maria,
Sies Helmut
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80524-5
Subject(s) - glutathione disulfide , antimycin a , chemistry , glutathione , medicine , electron transport chain , biochemistry , enzyme
The relationship between the rate of glutathione disulfide (GSSG) export and the energy state was studied in isolated perfused rat heart. The intracellular GSSG level was maintained at saturation for transport (7.5 nmol GSSG · min −1 · g heart −1 ) by continuous perfusion with 20 μM t ‐butyl hydroperoxide. GSSG release was substantially restricted upon the addition of inhibitors of mitochondrial respiration such as KCN, antimycin A or rotenone. In contrast, no effect was observed on GSSG release during potassium‐induced cardiac arrest, although changes in oxygen consumption and coronary flow were similar to those observed with KCN. The dependence of the GSSG transport rate on the cytosolic free ATP/ADP ratio reveals that GSSG transport is half‐maximal at (ATP/ADP) free ≈ 10. The capacity of GSSG transport was unchanged by infusion of epinephrine, norepinephrine or dibutyryl cyclic AMP.