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The structure of C hordein
Author(s) -
Field J.M.,
Tatham A.S.,
Baker A.M.,
Shewry P.R.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80514-2
Subject(s) - hordein , circular dichroism , chemistry , protein secondary structure , molecule , crystallography , chromatography , storage protein , organic chemistry , biochemistry , gene
The intrinsic viscosity of C hordein was determined in 70% ( ) aqueous ethanol (± 10 mM NaCl) and 0.1 M acetic acid at temperatures between 15.3 and 50°C. Calculations based on the assumption (supported by previously published results) that C hordein has an ordered conformation indicated that the molecule is rod shaped, with molecular dimensions varying from about 360 × 17 Å to 265 × 20 Å. Circular dichroism spectroscopy indicated a secondary structure rich in β‐turns, with some conformational differences in the two solvents and at different temperatures. The results are compatible with C hordein having a helical secondary structure based on repetitive β‐turns.