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Oxygen dependence of vitamin K‐dependent carboxylase and vitamin K epoxidase
Author(s) -
McTigue John J.,
Suttie J.W.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80513-0
Subject(s) - pyruvate carboxylase , vitamin , enzyme , dithiothreitol , chemistry , biochemistry , oxygen , substrate (aquarium) , oxygenase , biology , organic chemistry , ecology
A study of the oxygen requirements of the rat liver microsomal vitamin K‐dependent carboxylase and vitamin K 2,3‐epoxidase indicated that both enzymes had a K m for O 2 in the range 60–80 μM. This value was not influenced by vitamin concentration, alterations in carboxylase substrate, Mn 2+ , or dithiothreitol, and is consistent with the hypothesis that both activities are catalyzed by the same enzyme.