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Glucose reduces both Rb + influx and efflux in pancreatic islet cells
Author(s) -
Lindström Per,
Norlund Lena,
Sehlin Janove
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80512-9
Subject(s) - islet , efflux , chemistry , pancreatic islets , medicine , endocrinology , biochemistry , biology , diabetes mellitus
Microdissected, β‐cell‐rich pancreatic islets from ob/ob mice were used in studies of 86 Rb + transport. D‐Glucose (20 mM) induced a biphasic reduction in 86 R + efflux. The reduction stabilized within 10 min at 34% of the efflux rate at zero glucose. The initial 86 Rb + uptake (5 min) was dose‐dependently reduced by ouabain with maximum inhibition at 1 mM. D‐Glucose (20 mM) did not affect the ouabain‐sensitive 86 Rb + influx but markedly reduced (48%) the ouabain‐resistant isotope influx. The results suggest that D‐glucose does not affect the Na + /K + pump in pancreatic β‐cells and that the glucose‐sensitive K + ‐transporting modalities (K + channels) in the β‐cells can mediate both inward and outward K + flux.