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Requirement for bivalent cations in the actions of insulin and sodium nitroprusside on metabolism in rat adipocytes
Author(s) -
Emami Shahin,
Perry Michael C.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80509-9
Subject(s) - sodium nitroprusside , lipolysis , medicine , endocrinology , chemistry , insulin , sodium , metabolism , adipocyte , triglyceride , biochemistry , adipose tissue , nitric oxide , biology , cholesterol , organic chemistry
The requirement for Ca 2+ and Mg 2+ in the actions of insulin and sodium nitroprusside on rat adipocyte metabolism was investigated: (i) sodium nitroprusside, but not insulin, increased cGMP levels in cells incubated in the absence of Ca 2+ and/or Mg 2+ ; (ii) sodium nitroprusside and insulin are unable to increase the incorporation of [ 14 C]glucose into triglycerides and [ 14 C]leucine into proteins in the absence of Ca 2+ and Mg 2+ ; (iii) sodium nitroprusside and insulin showed antilipolytic actions in Ca 2+ ‐ and Mg 2+ ‐free medium. We conclude that in the absence of Ca 2+ and Mg 2+ , sodium nitroprusside and insulin have very similar regulatory properties on triglyceride, protein synthesis and adrenaline‐stimulated lipolysis, but not on cGMP levels in rat adipocytes. This could provide evidence that omission of bivalent cations was inhibitory at more than one site, or that sodium nitroprusside mimics insulin's actions by another mechanism that does not involve cGMP.